Stabilization of virus-like particles (VLP)

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juichin
juichin's picture
Stabilization of virus-like particles (VLP)

Dear colleaques,
 
I has anyone an idea with additives I can use to stabilize purified virus-like particles (VLP)?
 
Thanks for your help.

Jason King
Jason King's picture
 

 
Which virus are the VLPs based on?

kjosephs
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I think you will get more

I think you will get more specific answers if you say a little more about what type of particles you are working with, a little bit about how they are prepared and what you observed that makes you think your VLPs need stabilization.
Buffer components I have seen used to stablilize proteins and protein complexes include the following:
optimized buffer pH
optimized salt concentration
glycerol up to 50%,
tween 20 up to 0.5%,
BSA,
BME, DTT, TCEP, 
Arginine
Sucrose
Note that components like buffers and salts are not all equivalent so it can be necessary to vary these as well. For example, use Hepes instead of Phosphate to buffer at pH 7.0, or KCl instead of NaCl.

juichin
juichin's picture
Dear colleaques,

Dear colleaques,

I has anyone an idea with additives I can use to stabilize purified virus-like particles (VLP)?
All VLP are based on core protein of Hepatitis B virus (HBcAg).

Thanks for your help.

Jason King
Jason King's picture
juichin wrote:

juichin wrote:

Dear colleaques,

I has anyone an idea with additives I can use to stabilize purified virus-like particles (VLP)?
All VLP are based on core protein of Hepatitis B virus (HBcAg).

Thanks for your help.

 
Did the VLPs bud from the euk. producing cells and thus aquire an envelope or are they just C protein assemblies ? And do they contain either RNA (premature) or DNA within the capsid or non-specific nucleic acids (eg stemming from a self-assembly from E.coli extracts)?