Glad to find this forum with so many useful information.
I am working a protein adduct characterizing project. I was able to seperate the target protein by a RP HPLC (C4 column, Solvent A: water with 0.1% TFA; B Acetonitrile with 0.1 TFA), gradient 30%B to 95%B.
However, the adduct seems not stable under acidic condition. I am thinking if it is possible to use some netural solvent system to isolate my protein? Thanks.