I am purifying GST fusion protein but i didn't get fusion protein. After IPTG induction my protein expressed well. i got good band with target size..but after purify fusion protein, i got vector size only in elution..There was no fusion protein band. I used Glutathione HiCap Matrix Kit system..i dont know where my fusion protein went..pls give me some suggestion...
I request you to please help me..
Looking for your kind reply..
Thanks in Advance...
With Kind Regards