Question about GST fusion protein purification

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biovinz
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Question about GST fusion protein purification

 Hi all,
    I am purifying GST fusion protein but i didn't get fusion protein. After IPTG induction my protein expressed well. i got good band with target size..but after purify fusion protein, i got vector size only in elution..There was no fusion protein band. I used Glutathione HiCap Matrix Kit system..i dont know where my fusion protein went..pls give me some suggestion...

I request you to please help me..

Looking for your kind reply..

Thanks in Advance...

With Kind Regards
VINOD