purification using DEAE cellulose

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ranbir
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purification using DEAE cellulose

Hi friends, i'm working on purification of antifungal protein. How much protein should one load on DEAE column of 1 ml capacity?

Sami Tuomivaara
Sami Tuomivaara's picture
Ranbir,

Ranbir,

The column (or resin) comes with a brochure which should state the capacity, alternatively, you should visit the manufacturer's webpage to obtain the information. For example, Whatman DEAE resins have 50 - 150 mg total protein/ml resin capacity. Word of warning though, usually the manufacturers' report the capacity for some (pure) high-binding protein, so it is probably overestimate for crude protein mixture.

Cheers,