I am trying to express protein in PQE30(qiagen). there is no desired protein at all. i used a protocol which involved washes with 0.5M Nacl+2% triton X 100 ,Washes with 0.5mNacl, followed with sterile water. is this the correct method? I repeated this for thrice same result.
i am frustated with this. can anybody suggest me what to do?
thanks in advance.