i started this topic in the title"protein isolation in pqe-30 vector".
i can't go for western. the washing steps procedure was estabilished for pEt29 vector. i used the same here. but no protein.
when i checked cell lysates ( harvested cells + lysis buffer+ sonicaton), i find the 130kd band in uninduced , induced ones.still, there is no change in intensity of band.
i have used Xl-blue cells as host.
can any one suggest a method to get protein.