Protein Inhibitors (fast acting)

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TRMADR
TRMADR's picture
Protein Inhibitors (fast acting)

I am interested in finding a fast acting protein synthesis inhibitors for cell culture studies. I am familiar with some inhibitors (anisomycin, cycloheximide), but these are slow for uptake by cells. I want to use a fast-acting inhibitor for B cell culture that are treated with TLR ligands. The idea is to prevent any protein synthesis during the treatment. Does anyone have any suggestions? Thanks in advance!

Fraser Moss
Fraser Moss's picture
Apparently inducing Hypoxia

Apparently inducing Hypoxia can cause fast acting and reversible inhibition of protein synthesis

[url=http://mcb.asm.org/cgi/content/full/22/21/7405?maxtoshow=&HITS=10&hits=1... of Protein Synthesis by Hypoxia via Activation of the Endoplasmic HWFIG]RegulationReticulum Kinase PERK and Phosphorylation of the Translation Initiation Factor eIF2{alpha}[/url]

Fraser Moss
Fraser Moss's picture
Here's an alternative