I am working with isolation and purification of protein of around 6kD produced in E.coli.
I am facing problem in its analysis i.e after IEX when I am running samples on SDS page I could see bands corresponding to size of my protein of interest. But when i am submitting samples for RP-HPLC analysis using C-18 column, I am not able detect protein of my interest. Where as standard / innovator samples appears to be fine in both the cases.
can anyone share experiences/ soultions