Protease required!!!!

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juichin
juichin's picture
Protease required!!!!

Dear Scientists and colleagues,

Im looking for a protease (every host is welcome) which cleaves specific at the N-terminus of its protease cleavage site, resulting in no additional amino acid residue (aa) at the C-terminus of target after cleavage (P1` site is the first aa of cleavage site and P1 is final C- terminal aa of target protein(...P3P2P1/P1P2P3...)).
Has anyone an idea where such protease is available, or does anyone know a host (vegetable, viral, mammalian or bacterial origin) producing such protease with this feature?

Thanks for your collaboration and for your efforts,
Best, MK.

cam1bmf
cam1bmf's picture
Hello, I have heard of Lys-N

Hello, I have heard of Lys-N It was recently published in a paper Nature Methods May 2008 Vol 5 No 5 One of the Authors was Shabaz Mohammed.

As far as the availability of Lys-N I think you can only get it from Japan

Protea BioSciences www.proteabio.com carries a wide selection of different Proteases

Hope this helps,

Cam

R Bishop
R Bishop's picture
Lys-N is also known as

Lys-N is also known as Armillaria mellea neutral proteinase or Peptidyl-Lys metalloendopeptidase from Grifola frondosa and can be obtained from Seikagaku Corp in Japan. They sell in the US through Cape Cod Associates.

Good luck

Rb

Qingsong
Qingsong's picture
I can not get the catalog

I can not get the catalog number of Lys-N, please send me a detailed infromation about Lys-N in Seikagaku, thanks very much! My email address is :wangqingsong@gmail.com

R Bishop
R Bishop's picture
Qingsong,

Qingsong,
I had a hard time finding Lys-N again as well.Cape Cod no longer carries it.
 
Lys-C from sigma is here though and might help if you are looking to cleave next to lysines.
www.sigmaaldrich.com/catalog/ProductDetail.do
 

qiang wang
qiang wang's picture
I can't find it either, but

I can't find it either, but Dr Miyagi had some publications with Lys-N perchased from Seikagaku, you may want to email him and ask for the catalog #

Masaru Miyagi, Ph.D.
Department of Biochemistry and Molecular Biology
University of North Dakota School of Medicine
501 North Columbia Rd.
Grand Forks, ND 58203
Tel: (701) 777-2760
Fax: (701) 777-2382
E-mail: eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%6d%6d%69%79%61%67%69%40%6d%65%64%69%63%69%6e%65%2e%6e%6f%64%61%6b%2e%65%64%75%22%3e%6d%6d%69%79%61%67%69%40%6d%65%64%69%63%69%6e%65%2e%6e%6f%64%61%6b%2e%65%64%75%3c%2f%61%3e%27%29%3b'))