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We are currently performing a Blue Native gel electrophoresis to visualize intact mitochondrial complexes from mice heart following the method published by Schagger´s group (Nature Protocols 1(1): 418-428, 2006). We have tried to do it in both mitochondrial crude fraction and mitochondrial enriched fraction using Dodecylmaltoside as detergent and Comassie Blue G-250. For the purpose, we are using PhastSystem Separation-Control and Development Units 220 VAC (18-1018-24), PhastGel Gradient – 4-15 (17-0678-01) and PhastGel Buffer Strips – Native (17-0517-01) from GE. The method that we are using is the pre-saved method 1 called “Native” in the PhastSystem Separation Unit. However, we are experimenting a big problem: when the sample is running under step 3 (around 70-75 AVh) a drop in the mA is experimented (from 10 mA to 0.4 mA; 400 V and 0 W). With this level of mA the electrophoresis is almost stopped on half-2/3 of the gel. I don´t understand the reason of this problem. Do you have any idea?