nuclear protein extraction

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leila
leila's picture
nuclear protein extraction

i need to about 50 µg nuclear protein to assay nf kapaab in h9c2 cells  and the growth rate of that is very  slow  . I have only 500000 cells in each 75 flask I use Eliza cayman kit but for that  the yield of my pr is very low . i would be appreciate  if I can use your experience.