Looking for specific protein ladder

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S.A.D.
S.A.D.'s picture
Looking for specific protein ladder

Hello,

I am currently dealing with 4 isoforms of a given protein, and their molecular weights are roughly 122, 130, 132, 137 kDa respectively.

Most of the standards/ ladders that I have come across usually have one marker at ~100kDa and then jump straight to either ~150kDa or ~200kDa, skipping my entire range of interest.

Anyone know of any size standards/ ladders available that will help resolve these isoforms for me in the range of about 120 to 140 kDa?

Thanks a lot

Fraser Moss
Fraser Moss's picture
you could combine these two

you could combine these two ladders

Fermenas PageRuler™ Prestained Protein Ladder, #SM0671/2
http://www.fermentas.com/catalog/electrophoresis/marksm0671.htm
has 100, 130 170 kDa bands

BenchMark Prestained Protein Ladder
http://tinyurl.com/5cmwoo
85, 120, 190 kDa

then al least you'll have the 120-130 kDa boundaries marked and if you run out the gel enough you should be able to resolve the small differences between each sample.

mchinmoyee
mchinmoyee's picture
You can run a gel with 2-3

You can run a gel with 2-3 markers in different well or if different colors can mix and run in the same well having variety of combo for your purpose.
Since you want to detect 122, 130, 132, 137 kDa proteins
the following makers have 120,130 & 140 so you can use them in combo in low percenage gel.

Fermenas PageRuler™ Prestained Protein Ladder #SM0671
100, 130 170 kDa bands at higher KDa

BenchMark Prestained Protein Ladder # 10748-010 Invitrogen
85, 120, 190 kDa at higher KDa

NexusView 20kDa Dual Color have # Code: BNPM20 Bionexus
100, 120, 140 kDa at higher KDa

Good Luck

S.A.D.
S.A.D.'s picture
Thank you for your reply.

Thank you for your reply.

I just realised though, which I probably should have mentioned before, in our lab we only have 10% Bis-Tris gels, and we use MOPS-SDS as the running buffer, so even though your suggestions were great, unfortunately they didnt fit with the particular gel system that I am running at the moment, sorry I should have mentioned that before.

Anyways, if you know a good pre-stained protein ladder for Bis-Tris gels, and MOPS as running buffer please let me know. And the range I'm interested in is between 120 to 140 kDa, the more markers in this range - the better

Thank you for all your help

mchinmoyee wrote:

You can run a gel with 2-3 markers in different well or if different colors can mix and run in the same well having variety of combo for your purpose.
Since you want to detect 122, 130, 132, 137 kDa proteins
the following makers have 120,130 & 140 so you can use them in combo in low percenage gel.

Fermenas PageRuler™ Prestained Protein Ladder #SM0671
100, 130 170 kDa bands at higher KDa

BenchMark Prestained Protein Ladder # 10748-010 Invitrogen
85, 120, 190 kDa at higher KDa

NexusView 20kDa Dual Color have # Code: BNPM20 Bionexus
100, 120, 140 kDa at higher KDa

Good Luck