OK, i'm not sure if this is the right forum for this - the choices seem a bit confusing; so if a moderator moves this thread to another forum; THANKS!
Anyway, I am looking for some tips / feedback on how you successfully pull down huge proteins in an IP. I'm working with a 220kd protein that binds many secondary factors. So far, I can western this protein from whole cell lysates without much trouble, but my IPs are aweful. I've tried 3 different antibodies for the same protein thus far, but it's been 4 months now and my IPs continue to show very little of my target protein is getting pulled down.
any tips you might have would be very much appreciated.