I want to seprate the human serum albumin and Apo-2L from lyophilized powder, and i can do it very well 4 years ago. But the human serum albumin appeared peak bifurcating in the same chromatographic conditions in recent years.
And then i tested the titerof my column, and the plates is 17000, it is showed that the state of my column is good. Then i contact factory of my column,they said it is the problem of my samples,
but the sigma product (pure albumin of human serum) and the baxter albumin product also appeared the peak bifurcating. i don't know if it is because the albumin have the isomers in the two products.
my chromatographic conditons : tempreture 40℃, Mobile phase:acetonitrile and H2O(0.01%TFA).
someone please help me why the peak bifurcate? Thank you very much!