I'm shopping for Phenylmethanesulfonyl fluoride (PMSF) on the sigma web site
I need to include 1mM in my resuspension solution for my cells before I lyse them to purify my expressed protein.
I see that Sigma sell a 0.1M stock in ethanol. PMSF is very unstable in water but will the 1part in 100 of ethanol that would result in my working solution if I diluted this into by resuspension buffer have an adverse effect on the cell prep or protein that I will purify?
Also In what buffer do you dissolve your stock PMSF and how do you store it?