Can I overdry proteins?

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Tiffy
Tiffy's picture
Can I overdry proteins?

Hello,

I have a question about protein precipitation. I precipitate my proteins with methanol and chloroform. The final step is that I wash my pellets with methanol (1ml). Because of the fragility of the pellet I put it in all in the speed vac and dry the pellet. What could happen with the proteins when I overdry the pellet. After drying I resuspend the pellet with 2x sample buffer. When I loaded the protein on the SDS-PAGE I observed that in the bottom of the tube a residue is visible. Something viscous or the like. Is this the overdried pellet and the proof that not everything was properly resuspended?
Thanks for all comments.
Regards Tiffy

pguo
pguo's picture
No, You can't. You will lose

No, You can't. You will lose the antigens if you did so. Here is some points you can refer. http://www.e-biotek.com/
Good luck with your expts.