I have purified an IgG using AKTA/FPLC and ran the purified product on HPLC. I need to calculate how much antibody I have from the Peak Area (which is in mAu*min). I know the flow rate but I don't have the extinction coefficient for my IgG. I guess I can estimate the extinction coefficient for IgG. But can someone please tell me How I can calculate it using the arithmetic. Thanks a lot.
Calculating Antibody concentration from HPLC chromatogram