best leader peptide for periplasmic expression

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juichin
juichin's picture
best leader peptide for periplasmic expression

Hey,

Im looking for a leader peptide (native or synthetic origin) for high yield expression, improved translocation across the cytoplasmic membrane and enhanced SPase-I proteolysis. Has anyone experiences which leader peptide is suitable for this approach? Numbers of studies used synthetic leader peptides. It seems to be interesting.

Thanks for your help,

Best MKr