Setting up MDR1-MDCK assay

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firstlast2929
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Setting up MDR1-MDCK assay

Hi all,

I'm trying to setup MDR1-MDCK assay. I have lots of literature but most seem to be lacking a detailed procedure.

I know most start at 10um test compound. However what is volume of buffer in the 24 well plates? Any help would be greatly apprecaited!