GTPgammaS Binding Assay

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aif
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GTPgammaS Binding Assay

Hi all!

I've been doing this assay for several months, and repeatedly I get high results and no response to the agonist (glutamate). Other times it works properly.
All the conditions were optimized. I use frozen membranes from CHO cells expressing mGlu2 receptors.
What do you think can be causing these results? Regarding the thawing and dilution of the membranes, which I think that can be related to my problem, do you have some advice you think is important?

Thank you,
A.