I am trying setup an extracellular rig to study the CA1 LTP.
I have one old set up was not working for many years and trying put back things to work.
I have Axopatch 200A amplifier, Grass S88 stimulator, an lab view acquisition program.
But I have the following issues with that set up.
Initially I was using interface chamber , where I could not maintain the proper bath level, when the high bath level, response is low and when I reduce the level its becomes better. But after that the fEPSP amplitude started increasing, looks like my slice is drying..
Now I changed to submerged chamber, then If I keep high bath level again the response is low and I have to reduce the bath level here too. I am using temperature controller to maintain the chamber but its not going beyond 26 C. Is it because of the temprature I am not getting proper response or any other problem in the setup? The over all gain is 500, Low pass filter is 10kHz, I sthere anything with the settings I have to check ?
I have lot of DC shifts and the baseline is not stable.
I am not sure about my recoding electrode resistance, how do I measure the resistance?
Please help me to trouble shoot the issue
Hippocampal CA1 field recording