What I want to do seems pretty hard:
I want to do visually guided patch clamp in brain slices sitting on our microelectrode arrays, which are not transparent. So I'm trying to find illumination techinques that do not need transparent surface (unlike IR-DIC)... I could illuminate from the side maybe, but I don't think I will see anything in the tissue...
If anybody has experience with that or any ideas, would be great!