Mature hippocampal slice culture

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JCF
JCF's picture
Mature hippocampal slice culture

Hello.

I'm looking for a protocol for culture of mature hippocampal slices. I'm not interested in organotypic slices culture, since I only need them to be in culture for 24 or 48 hours. If anyone knows, or has, one, please tell me.

Thank you advance...

 

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi JCF,

Hi JCF,

here is one paper which i hope will help you in solving your querry

" Culture of mature hippocampus slices for 4 days in a newly developed medium: preservation of transmitter release and leucine incorporation into protein "
www.sciencedirect.com/science

and if you are interested in long term culture of  mature hippocampal slices here is another paper
"Long-term maintenance of mature hippocampal slices in vitro"
www.sciencedirect.com/science
 


JCF
JCF's picture
Hello again.

Hello again.

Thank you very much for your help!