Hello friends, does anybody have any experience with using the LTP Assistant in pCLAMP 9/10 software (Axon Instruments) for stimulation during extracellular LTP recording? I have tried it but not able to do good. Which mode do you recommend for use in this case, with LTP assistant..........episodic or high speed oscilloscope mode? Also, is there any other way to give appropriate stimulation for baseline and tetanus stimulation protocols, without the use of a stimulator, ie by using only the pCLAMP software and the stimulus isolator? Please somebody help me out with these problems. I am completely stuck.