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orchidsbloom's picture

 Hello everyone,

I had a really long day in lab and ended up spending the night here. Among the several things I had to do, I did a yeast high-efficiency transformation using lithium acetate. I exactly followed the protocol, added all the reagents in the order specified, heat shocked for 40 min at 42 degrees, took them out and centrifuged them. Something else came my way and I completely forgot about the cells and fell asleep for three hours. After I woke up, I realised that my cell pellet was sitting for three hours with the transformation mix as the supernatant part. Is my transformation useless now? Please help... I really really would like to know. I did plate the cells anyways but even if i get colonies, I am not sure I should trust them now!  Please help!