using image J for IHC interpretation

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arwa
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using image J for IHC interpretation

Excuse me everyone, i want to know how to use image J program for quantifying positive cells stained by immunohistochemistry.

& should i make counting to all cells in the field & then counting of the positive cells next, to get accurate results??

& how i can make measuring for the intensity   " optical density " of the stain using the same program?

Thanks in advance.