I've been doing some multiphoton imaging of nanoparticles at a variety of wavelengths. I now have separate images of the same samples at three wavelengths and want to combine the images to show only the regions of colocalization.
However, I need the pixel contributions from each of the three channels to only be displayed if they're at a specified ratio to each other, i.e. each pixel in channel A that is 2x the intensity of the corresponding pixel in channel B is selected and all others are discarded etc.
I've seen an interesting plugin:
But I don't really know how to express the ratios as percentages. For instance, if I only want to display the green channel's pixels if they're 2x the intensity of the corresponding pixels in the red channel, how do I do that?
Does anyone here know how to help?