Hello! I am using Image J to find the area of mitochondria with axons. Using immunofluorescence mitochondria have been have been labelled and appear as the red channel. By analysing particles I can number the clusters of mitochondria - all good so far!
My problem is I would like to be able to calculate the area of a third channel with is a mitochondrial subunit within the red particles (mitochondrial networks) that I have already created when analysing the red channel data.
I hope this makes sense and any methods would be much appreciated!