I am looking for an inverted scope that will allow me to image fluorescently labeled cells on the surface of an opaque material. I am considering the Nikon TS100, but I was also wondering if anyone has used fluorescence with the Olympus CK40 scope.
Any references using this technique would be appreciated.
I am using Zeiss Axiovert 200M both for micromanipulations and Fluorescent observations. Works fine, except of few bugs in the sofware provided. If equipped with ApoTome module - allows to get pictures as good as those from confocal imaging.
I used to use Zeiss also.
It was a AxioVert200 I was very happy happy with it.
http://www.zeiss.com/4125681F004CA025/?Open
I used the Axiovision softwear which was great.
Guy
We currently use a Olympus IX81 frame with the DSU attached. It works great for imaging on Transwell filters - which are largely opaque. Again, the DSU allows z-axis segments - not as fine as LSM, but with no bleaching and a tenth of the price.
We have Slidebook and imagePro software to go with it - controls both microscope functions and image acquistion.