OD600

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saumyadeb
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OD600

 I prepared an E.coli culture in LB medium and made serial dilutions to enumerate CFU/mL using plate counting. According to my plate count I should have 10^8 CFU/mL in Tube No.1 (1mL of culture in 9mL of LB). However, when I take spectrophotometric readings using OD600, I get readings of the range of 0.02-0.03. I cant find any way to explain this.