cheaper CO2 generation

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flavobms's picture
cheaper CO2 generation

How i can generate Carbon dioxide gas in bacteriological incubator which doesnt have the co2 gas supplementation. what are steps i need to take to generate the gas

Arvind Singh Pundir
Arvind Singh Pundir's picture
people had tried generating

people had tried generating CO2 and H2 in the past but the thing is how will the percentage of CO2 will be controlled inside the incubator 
Nam Woong Yang and Yong Lim*
*Department of Microbiology, Chosun University, Medical School, Gwangju 501-759, Republic of Korea.
The purpose of this study was to develop a multipurpose incubator, without the gas cylinders (bottles) which are required for H2 and CO2 supplementation. In our bottle-free multipurpose incubator, the H2 and CO2 were generated by chemical reactions induced within the chamber. The reaction between sodium borohydride and acetic acid at a molar ratio of 1:1 was used to generate H2, according to the following formula: 4NaBH4 + 2CH3COOH + 7H2O --> 2CH3COONa + Na2B4(O7) + 16H2, whereas the other reaction, citric acid and sodium bicarbonate at a 1:1 molar ratio, was used to generate CO2, according to the following formula: C6H8(O7) + 3NaHCO3 --> Na3(C6H5(O7)) + 3H2O + 3CO2. Five species of obligate anaerobic bacteria, one strain of capnophilic bacterium, and one strain of microaerophilic bacterium were successfully cultured in the presence of their respective suitable conditions, all of which were successfully generated by our bottle-free multipurpose incubator. We conclude that, due to its greater safety, versatility, and significantly lower operating costs, this bottle-free multipurpose incubator can be used for the production of fastidious bacterial cultures, and constitutes a favorable step above existing anaerobic incubators.
The Journal of Microbiology, February 2005, p.28-33 Vol. 43, No. 1  

"Development of a bottle-free multipurpose incubator for generating various bacterial culture conditions"

flavobms's picture
thanks for your reply. i am

thanks for your reply. i am doing human lymphocyte culture from peripheral blood of human. can supplement the CO2 gas by using the method which is given in the paper.

g a
g a's picture
Cultuing with sodium pyruvate

Cultuing with sodium pyruvate eliminates the need for co2 as pyruvate acts as internal co2 supplier.

Arpit Kakkar
Arpit Kakkar's picture
biological lab equipments

Incubators are used in biological research work in applications like BOD Determinations, Plant and Insect Studies, Fermentation Studies, and Bacterial Culturing. BOD incubator manufacturers  keep in mind all things and design incubator which are easy to use. Possibly other incubator are used in co2 generation.