Understanding the regulation of lantibiotic production and resistance in the actinomycete - Microbispora
Our holistic approach integrates cellular physiology, molecular genetics (including functional genomics), biochemistry and structural biology. In the Department of Molecular Microbiology we study bacterial metabolism, physiology, gene regulation and development, and plant-bacterial interactions.
Major themes of our work on Gram-negative bacteria are the molecular basis of signal transduction in response to various environmental signals and studies on the biology of ammonia channel proteins which are conserved in all domains of life.
The Department's comprehensive Streptomyces programme, studying antibiotic production, morphological differentiation and stress responses, is underpinned by the newly-acquired genome sequence of the model species S. coelicolor, and cutting-edge techniques in functional genomics.
Another major research area is the Rhizobium-legume symbiosis where our studies range from bacterial quorum-sensing and plant-bacterial signalling interactions to the developmental responses of plants to their microbial symbionts.
The successful applicant for this position should have expertise in the genetic manipulation of actinomycetes. It is essential that candidates have a PhD in biological sciences (or have submitted, or are about to submit, their PhD thesis) together with an understanding of bacterial genetics and the regulation of bacterial gene expression. Knowledge of some/all of the following is desirable:
Experience in culturing and DNA isolation from actinomycetes and the control of secondary metabolism in streptomycetes, experience in carrying out growth curve experiments, RNA isolation, transcript mapping, qRT-PCR, EMSA, DNaseI protection analysis, in vitro assays, antibacterial activity bioassays, protein expression and purification, and analytical chemistry procedures (HPLC, LC-MS, FAB-MS, NMR). Good communication and organisational skills are required, together with the ability to work independently planning and executing specific experiments.
Closing Date 15 May 2011