Transwell

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sander164
sander164's picture
Transwell

I'm looking for a straightforward protocol to perform transwell experiments. I’m currently busy looking at suppression by regulatory T cells and if it is contact dependent or dependent on soluble factors.
 
Furthermore I would like to know with which manufacturer you have the best experience with respect to their transwells and which pore size you use.
 
Also, I’m planning to use CFSE to look at proliferation. But I have heard that using WST-1 is more convenient in a transwell experiment. Can anyone comment on that?
 
Thanks!

heehawmcduff
heehawmcduff's picture
Hi,

Hi,

Regarding your choice of transwells, I have used Corning in the past and their transwells have been excellent quality.  For monolayers of epithelial cells, I have used 0.4um pore size, but for neutrophil/macrophage migration studies, I used 3um and 5um pores respectively.