Structural analysis of bacteria

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angelaevan
angelaevan's picture
Structural analysis of bacteria

Hi,

We have been immunizing with live Rhodococcous equi (gram positive) to elicit an immune response, we show  that works in raising IgG levels for that antigen with Elisas.  Now we could not use the live R.equi and we "attenuated" it with UV irradiation (254nm)  we are getting good elisa results and have even done western blots of the serum comparing the serum from live antigen and the irradiated antigen and are getting similar bands.  But,  we need to show that the bacteria has remained structurally intact does anyone know how I can do this?

heehawmcduff
heehawmcduff's picture
Hi

Hi

I guess one straightforward way, if you have access to the facilities, would be direct imaging of the bacteria with electron microscopy or by fluorescent confocal microscopy.  If going the confocal route, you could pick a couple of antibodies directed to specific structures such as the cell wall.