I have been carrying out some preliminary work on Jurkat cells and the mitogens PMA/IoM and Concanavalin A. I incubed Jurkat cells with varying levels of PMA/IoM or Concanavalin A for 24 h, 48 h, 72 h and 96 h (today). The cell viability results have been coming out OK. All conditions are resulting in greater than 90% cell viability.
However, I have noticed that the cell numbers in the controls (cells only and carrier controls) are far greater than those in the test samples i.e. cell proliferation has been severely affected by the mitogens! Based on the literature, I was expecting the cells to grow well in the presence of the mitogens. Any ideas PLEASE?