PI staining

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vikramneuro
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PI staining

HI Friends,

as I am studying apoptosis in glial cells and using PI staining for the dead cells.

My question is should I gate my cells in FFC/SSC density plot to know the number of dead cells (PI positive) or not?

secondly Should I use log scale or linear scale for my histogram measurements or analysis?

thanks a lot for your help in advance!
regards
vikram