Macrophages

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katimani
katimani's picture
Macrophages

Hi,
I am trying to activate monocytes isolated from PBMC by using human serum AB ( 1X10e5 in 24-well plate). After 7 days, I can see the MDM, however, the cells are few in the plate, can we scrape the cells and plate them again so I can combine 2 to 3 wells in one well.  I want to use it for virus infection.

heehawmcduff
heehawmcduff's picture
Hi

Hi

I think it's certainly possible although you may see a small drop in viable cell numbers.  I have previously pooled peripheral blood monos from different wells for flow cytometry but have never replated them.  My best guess is that it should work fine.

Let us know how you get on.