lipids ultracentrifugation serum

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erg
erg's picture
lipids ultracentrifugation serum

Dear colleagues,
is there anyone who has worked in serology and has ultracentrifugate the serum in order to eliminate the lipids presents in? Which protocol have you followed?
Besides, I have the doubt that if I eliminate lipids it's possible that I eliminate the antibodies anti-lipids that could be in immunocomplexes too?
Thanks for your help
erg

dattak
dattak's picture
Hi!

Hi!
Generally, if you aliquot your serum into 1.5 or 2ml eppendorf tubes and spin them for 15-30 minutes at the highest speed of your micro-centrifuge (13.5-14K rpm for most), the lipids form a visible thin layer on top of the serum. With a pipette tip, you can carefully remove the serum from the side without disturbing the lipid layer.

As for immune complexes, it is almost impossible to dissociate immune complexes while keeping the antibodies intact.

Perhaps it would help if you point out the purpose for which you want to remove the lipids from your serum.

HTH. :)

erg
erg's picture
Hi!

Hi!
I'm working in serology. It has been describe that cholesterol could be detected by specific antibodies to other kind of lipids that I'm testing, so I'd eliminate the cross-interfence due to cholesterol. Could you help me?
Thanks in advance,

erg