ELISA troubles

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terrell's picture
ELISA troubles

Hello All,
We are experiencing difficulties with an established ELISA.  Saturated standard curves are occuring with all standards having nearly identical elevated OD values.  Using a 3% bovine albumin for blocking @ 1-2 hours and a human monoclonal IgG capture antibody for the standard curves and a HRP goat anti human IgG conjugate.  The other OD values on the plates are normal.  It seems as though the capture antibody and bovine albumin are cross reacting?  Thanks in advance for any help.