concentration of IL-2 in T cell proliferation

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sisterzero
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concentration of IL-2 in T cell proliferation

I want to do the assessment of T cell proliferation. I am not sure what dosage of IL-2(5ng/ul) should add into the culture media in 96-well plate.

Another quertion is how to convert ng/ul into IU/ml.

heehawmcduff
heehawmcduff's picture
For IL-2 therapy, I think

For IL-2 therapy, I think that the standard conversion of IL-2 concentrations into international units (IU) is as follows:
1ug = 18,000 IU

sisterzero
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 Thank you.

 Thank you.
I will have a try in this conversion.

sander164
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What concentration do you use

What concentration do you use for T cell activation? I use 50 U/ml. Which I refresh every 72h.

heehawmcduff
heehawmcduff's picture
That sounds good -  the

That sounds good -  the Miltenyi protocol I have calls for 20 units/ml changed every 3-4 days

(http://www.miltenyibiotec.com/download/datasheets/297/DS130-091-441.pdf)