I have a problem in doing bacterial agglutination titre of normal fish serum.
After 2 fold dilution in PBS in U shaped microtitre plate, I am adding 50 microlitres of formalin-killed Aeromonas hydrophila (OD- 2)...
This procedure was working previously, but now not working. Is there any problem in the plate...or bacteria.
Should I use sodium thiosulphate to wash the plate... or any other solutions please.