Bacterial agglutination of serum in micro titre plate method

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Pradipta Ranjan
Pradipta Ranjan's picture
Bacterial agglutination of serum in micro titre plate method

I have a problem in doing bacterial agglutination titre of normal fish serum. 

After 2 fold dilution in PBS in  U shaped microtitre plate, I am adding 50 microlitres of formalin-killed Aeromonas hydrophila (OD- 2)... 

This procedure was working previously, but now not working.  Is there any problem in the plate...or bacteria.

Should I use sodium thiosulphate to wash the plate... or any other solutions please.

heehawmcduff
heehawmcduff's picture
Hi there,

Hi there,

You said that your procedure worked previously.  Have you changed anything recently before it stopped working i.e. changed a supplier of serum or culture plates.

Also, are you using  a new stock of bacteria or one that has been through a large number of passages?