I'm new in the genomic field.
I know chandan_10_08 had posted a topic about the difference between EST and full-length cDNA.
However, I am still a bit confused on the differences between EST and "mRNA from GenBank" in UCSC genome browser.
1) the reliablity of a EST sequence is less than a mRNA sequence? EST was only sequenced once?
2) there are "human EST that have been spliced" and "human EST including unspliced". How is the splicing information of EST obtained?
3) Are the sequences listed in the "mRNA from GenBank" all full-length?