Simultaneous RNA and DNA extraction

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danica's picture
Simultaneous RNA and DNA extraction

Hi guys,

I want to isolate RNA and DNA from Anopheles mosquito samples. I have been trying different variations of the TRIzol protocol (back extraction buffer, sodium citrate) and have had little success in recovering good gDNA. My 260/230 ratios have generally been poor (0.30-0.60) and the absorbance peak at 270 nm! What's more puzzling is that when run on the gel, I get no bands!

I know there are some kits out there that can be a better alternative but I was wondering if anyone can recommend a particular kit from experience?

dws123's picture
Nice and informative thread

Nice and informative thread it is. Thank you all for sharing the information on HPLC assay method.
dna synthesizer

Nikail Collins
Nikail Collins's picture

There are several total nucleic extraction kits on the market.  I used to extract total nucleic acid from mosquitos using the EasyMag and the NucliSENS easyMAG, total nucleic acid extraction kit.  I am sure others work as well, but this is my experience.