Lysis buffer pH effectiveness

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AlBita's picture
Lysis buffer pH effectiveness

I am trying to isolate Genomic DNA from Algea. My problem is that I have low yield than what I am expecting from my cell pellet. I am using a lysis buffer composed of water, Tris-HCl pH8, NaCl, EDTA pH 8 2%SDS, Proteinase K, and 1% PVP. I am having trouble with aqueous and organic phase separation using phenol:chloroform, so I read that pH is important factor in effectiveness of lysis cells. I would like some help in preparing my lysis buffer at pH 9. I think my lysis buffer pH is 8 now. Can you give some suggestions.