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qPCR robotics

qPCR NEWS March 2008 - qPCR robotics

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is:

- New - qPCR robotics
- Update - RNA interference & small interfering RNA (siRNA)
- qPCR application workshops in 2008
- If this newsletter is not displayed correctly by your email client, please use following LINK: http://qpcrnews.gene-quantification.info/

qPCR robotics - http://qPCRrobotics.gene-quantification.info

Real-time quantitative PCR (qPCR) is now a mainstay of molecular biology. Just a few short years ago the purchase of a real-time instrument was considered a luxury. Now most laboratories either posses a real-time instrument or have easy access to one. We predict the same will happen for robotic automation of qPCR setup; it will soon be commonplace and probably expected.

A trend to automation is easy to understand. Firstly, the sheer numbers of reactions that are run (all with one or more replicates) means the workload is increasing dramatically. Secondly, and more importantly, qPCR setup requires great skill and lots of practice because even a very small variation when pipetting a DNA or RNA sample translates into big differences after amplification. There is also the question of repeatability. Identical results from different operators and between laboratories are expected but that can be very hard to achieve. Lets not forget that setting up qPCR assays is also tedious and time consuming! A small and affordable liquid handling robot with the precision necessary to tackle qPCR setup would address all these issues. The good news is robots like this are now available.

Choosing a robot - the essential attributes
The importance of robot software
qPCR applications using pipetting robots
Movie: PCR technology in BVDV diagnostics / molecular diagnostics / LIMS

... read more on ... http://qPCRrobotics.gene-quantification.info



Update - RNA interference (RNAi): siRNA, saRNA and microRNA
A lot of new RNAinterference papers using siRNA, saRNA and microRNA applications are present on various sub-pages.

RNA interference (RNAi) is a mechanism that inhibits or activates gene expression at the stage of translation or by hindering the transcription of specific genes. RNAi targets include RNA from viruses and transposons (a form of innate immune response), and also plays a role in regulating development and genome maintenance. Small interfering RNA strands (siRNA) are key to the RNAi process, and have complementary nucleotide sequences to the targeted RNA strand. Specific RNAi pathway proteins are guided by the siRNA to the targeted messenger RNA (mRNA), where they "cleave" the target, breaking it down into smaller portions that can no longer be translated into protein. A type of RNA transcribed from the genome itself, microRNA (miRNA), works in the same way.

RNA interference: hitting the ON switch. Researchers in San Francisco have findings that suggest a whole new side to RNA interference. Erika Check reports on their attempts to make a revolutionary field more revolutionary still.

Molecular biology: The expanding world of small RNAs
Molecular cell biology has long been dominated by a protein-centric view. But the emergence of small, non-coding RNAs challenges this perception. These plentiful RNAs regulate gene expression at different levels, and have essential roles in health and disease.

... read more on ... http://RNAinterference.gene-quantification.info/


update - microRNA

A lot of new papers and database links around microRNA are present on 5 pages.



With the new qPCR INFO PORTAL and all the presented tools we will help you with to find the right information about qPCR and related topics in Molecular Biology in the literature and in the World Wide Web.
=> Papers / Protocols / Methods / Databases / Alets / Feeds / Books / Forums / E-mail / Directory



Upcoming Events World-wide academic and commercial qPCR Events

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars, qPCR Education Program, ...etc...
Please submit your qPCR event here => eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%65%76%65%6e%74%73%40%67%65%6e%65%2d%71%75%61%6e%74%69%66%69%63%61%74%69%6f%6e%2e%69%6e%66%6f%22%3e%65%76%65%6e%74%73%40%67%65%6e%65%2d%71%75%61%6e%74%69%66%69%63%61%74%69%6f%6e%2e%69%6e%66%6f%3c%2f%61%3e%27%29%3b'))



TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, the 3-day Core Module and a 2-day Biostatistics Module. qPCR courses are held in regularly in Gteborg, Sweden, in English and in Freising-Weihenstephan, Germany, in German and English, and in Prague, Czech Republic in English and Czech.
Depending on the occasion the workshop language and the different prices may apply. Further customized workshops and specialized trainings will be held as well across Europe and world-wide. TATAA Biocenter Germany courses are held in cooperation with the Institute of Physiology, located at the Technical University of Munich, in Freising-Weihenstephan, near Munich, very close to the Munich Airport (MUC). For more information and to register for the qPCR application workshops, please see our web page: http://tataa.gene-quantification.info/

Course Occasions 2008:
3-day qPCR Core Module (Mon. - Wed.) and 2-day BioStatistics Module (Thu. - Fri.)

* 5 - 9th May 2008 (in Freising, Germany, Kurs wird in DEUTSCH gehalten, German language)
* 7 - 11th July 2008 (in Freising, Germany, English language)

Please register here => http://www.tataa.com/Courses/Courses.html


Forward Please send the qPCR NEWS to further scientists and friends who are interested in qPCR !

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages


If this newsletter is not displayed correctly by your email client, please use following LINK: http://qpcrnews.gene-quantification.info/
The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl and powered by BioScience Events. Copyright 2005 - 2008 All rights reserved. Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. Disclaimer & Copyrights are displayed on the homepage www.gene-quantification.com To subscribe or change your e-mail address in qPCR NEWS, and if you would like to receive future issues FREE of charge, please send an e-mail with the subject SUBSCRIBE to mailto:newsletter@gene-quantification.info?subject=SUBSCRIBE