Protein expression

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DinaLida's picture
Protein expression

To produce an antiserum, I am going to express the coat protein (CP) of a plant virus in E. coli using the pMAL protein fusion and purification system (New England Biolabs). When I insert the CP fragment in the expression vector, the protein will have nine additional amino acids in the amino end. How could I know if these additional amino acids may affect the protein expression or the antiserum production? What should I do in this case?
Thank you in advance.