DNA library ligation problems

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Sci-gal-92's picture
DNA library ligation problems

 Hi all,

I am working on building a DNA library, and have run into some problems. 

I believe that I have a problem during ligation of adapters. The final DNA is supposed to appear at 300/400bp, yet I always get a band at less than 100bp as well. Oftentimes, I do not get a band at 300-400bp at all. Can anyone tell me why this may be?