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vasu90's picture

 im working on two rat cell lines-ins1e cell line and RIN m5F cellline.i have tried reverse transcription pcr using rna isolated from RINm5F cellline .correct size bands were obtained for 4 genes. when i do the same using INS 1e cell line , band  were obtained at  double the size of actual size....i cannt figure out..plz help....

NOTE: I have checked the primer binding..and its all showing correct size...