I have started getting this ladder-like artifact in my images. I have attached 3 images that show this problem. I was wondering if any of you has seen it before and if so what is causing it. Also how can I get rid of it.
All this started very suddenly. I have been attempting to optimize this pcr assay for a few months now and it was working well. My primers are specific for my target and the yield is good.
Please can anybody help!
Ladder-effect seen in my pcr images!