Best Real time PCR instrument for multiplexing

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ccisotope
ccisotope's picture
Best Real time PCR instrument for multiplexing

Any opinions on the best real time instrument for multiplexing? Currently have Cepheid's smartcycler 1 and looking into upgrading to smartcycler 2 or ABI 7500 or 7900. Is Stratagene's instrument any good for multiplexing?

Thanks in adavance.

smartee
smartee's picture
Stratagene's Mx3000 is great.

Stratagene's Mx3000 is great. I've used one for the past year and half, and it's way better than anything I've worked on before, like the iCycler, ABI instruments, and MJR cyclers.

pharmacogenomics
pharmacogenomics's picture
You can check Rotor-Gene

You can check Rotor-Gene (Corbett Reserch) for real-time PCR. They have even 6 channels so you can amplify 5 target genes and 1 control together in one tube. Some modifications of Rotor-Gene have HRM function (high resolution melting) which could be applied for detection mutation, for instance.

Tony Rook
Tony Rook's picture
ccisotope:

ccisotope:

One thing you might want to evaluate before making the purchase is the software that comes with the real time thermocyclers. In my experience this is something that I overlooked and regret that I did. Make sure the software does not demand large setup times for each run and that it exports data in a format that easy to handle.

Real Time PCR
Real Time PCR's picture
One important criterion is

One important criterion is the choice of primer-probe design software that comes as a package with these instruments. Stratagene MX 300 and 4000 instruments bundle the industry standard Beacon Designer software. ABI bundles PrimerExpress while Bio-Rad again finds Beacon Designer as the instrument of choice for real time pcr assay design.

calvinsaeed
calvinsaeed's picture
Hello,

Hello,
I am looking for the best Real Time Instrument available in market for multiplexing. Anybody kind enough, mail me at eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%22%3e%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%3c%2f%61%3e%27%29%3b'))

Regards,
Calvin

Arvind Singh Pundir
Arvind Singh Pundir's picture
calvinsaeed wrote:Hello, I am

calvinsaeed wrote:

Hello, I am looking for the best Real Time Instrument available in market for multiplexing. Anybody kind enough, mail me at eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%22%3e%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%3c%2f%61%3e%27%29%3b')) Regards, Calvin

STRATAGENE is the best we have it in our Instt. for the past years and works best else go to the reviews on the all available qpcr instruments in market through the link below
www.gene-quantification.de/platform.html

Touchstone42
Touchstone42's picture
 An independent comparison of

 An independent comparison of qPCR machines is available at http://www.horizonpress.com/pcr/qPCR-machines.html

DR.Taylor
DR.Taylor's picture
 Does anyone know of a good

 Does anyone know of a good PCR machine for DNA sexing avian species?

Nikail Collins
Nikail Collins's picture
multiplexing

I have used ABI 7500, Smartcycler, and Lightcycler for real-time analysis using both monoplexing and multiplexing.  All are good. The development of the assay including primer, probes, and mastermix as well as cycling conditions is more important.

John Lee
John Lee's picture
DNA test equipment for bird sexing

I am really keen to find out any equiments of DNA test for bird sexing. Anyone out there please could help me on this.

Ivan Delgado
Ivan Delgado's picture
DNA test for bird sexing

Any real time PCR instrument, or even standard PCR instrument, will work for you. What you need is the actual sex-determination assay to run on any of these instruments. There are some commercially available assays you can buy, and you should also be able to find published assays based on genes like CHD and MHM.Good luck.