Best Real time PCR instrument for multiplexing

Stratagene's Mx3000 is great. I've used one for the past year and half, and it's way better than anything I've worked on before, like the iCycler, ABI instruments, and MJR cyclers.

You can check Rotor-Gene (Corbett Reserch) for real-time PCR. They have even 6 channels so you can amplify 5 target genes and 1 control together in one tube. Some modifications of Rotor-Gene have HRM function (high resolution melting) which could be applied for detection mutation, for instance.

ccisotope:

One thing you might want to evaluate before making the purchase is the software that comes with the real time thermocyclers. In my experience this is something that I overlooked and regret that I did. Make sure the software does not demand large setup times for each run and that it exports data in a format that easy to handle.

One important criterion is the choice of primer-probe design software that comes as a package with these instruments. Stratagene MX 300 and 4000 instruments bundle the industry standard Beacon Designer software. ABI bundles PrimerExpress while Bio-Rad again finds Beacon Designer as the instrument of choice for real time pcr assay design.

Hello,
I am looking for the best Real Time Instrument available in market for multiplexing. Anybody kind enough, mail me at eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%22%3e%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%3c%2f%61%3e%27%29%3b'))

Regards,
Calvin

calvinsaeed wrote:

Hello, I am looking for the best Real Time Instrument available in market for multiplexing. Anybody kind enough, mail me at eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%22%3e%63%61%6c%76%69%6e%2e%61%6b%68%74%65%72%40%61%6b%75%2e%65%64%75%3c%2f%61%3e%27%29%3b')) Regards, Calvin

STRATAGENE is the best we have it in our Instt. for the past years and works best else go to the reviews on the all available qpcr instruments in market through the link below
www.gene-quantification.de/platform.html

 An independent comparison of qPCR machines is available at http://www.horizonpress.com/pcr/qPCR-machines.html

 Does anyone know of a good PCR machine for DNA sexing avian species?

I am really keen to find out any equiments of DNA test for bird sexing. Anyone out there please could help me on this.

Any real time PCR instrument, or even standard PCR instrument, will work for you. What you need is the actual sex-determination assay to run on any of these instruments. There are some commercially available assays you can buy, and you should also be able to find published assays based on genes like CHD and MHM.Good luck.